| Tissue Preparation |
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ThermoShandon Excelsior Automatic Tissue Processor
Fully programmable and automated. Holds up to 222 cassettes using an organized basket or 300 cassettes using a random basket. Performs fixation, dehydrates, clears, and inflitrates with paraffin overnight. Quality of alcohol is tested before run and alerts the user if it needs to be replaced. This processor performs an even distribution of reagents resulting in high processing consistancy. Continuous agitation with this circular design allows a greater temperature uniformity. Can be operated by trained OMRF, OUHSC, or VA personnel
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RMC 1530 Automatic Tissue Processor
The Renaissance Automatic Tissue Processor (Ventana) can handle up to 350 tissue cassettes per run using any of 13 independently programmable user defined protocols. Performs fixation, dehydrates, clears, and inflitrates with paraffin overnight. Completed samples can be independently oriented and embedded in paraffin blocks using the Leica EG1160 Paraffin Embedding Center. Minimal training is required to operate the tissue processor and embedding center. For an additional fee, imaging facility personnel can be hired to operate the equipment. To insure availability, please make a reservation on the imaging facility calendar. If you need to cancel, you must notify imaging personnel before your scheduled run.
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Leica EG1160 Paraffin Embedder
The embedding center (used in conjuction with the paraffin processor) contains both a heated workstation and a cold plate for solidification of the sample. An integrated paraffin dispenser allows for continuous availability of liquid paraffin |
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ThermoShandon Consul Automatic Coverslipper
The Shandon Consul is a self-contained automated coverslipper, capable of coverslipping a maximum of 48 slides in less than 3 minutes per run. The coverslipper can be adapted to use coverslip sizes ranging from 22mm square to 24x60mm square, #1 or #1.5 (0.08-0.18mm) coverglass. The coverslipper is only available for slides requiring xylene-based mounting media. This coverslipper minimizes xylene exposure with an integrated activated-charcoal filter. |
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Shandon Finesse E Microtome
Designed for fully motorized sectioning of paraffin and resin-embedded specimens. Sectioning thickness is adjustable from 0.5 - 100 microns. The Coarse Advanced Wheel allows manual adjustment of sectioning thickness. The AUTOLOAD feature allows the user to save a ready position for quick block switching. Can be operated by trained OMRF, OUHSC, or VA personnel. Use of this equipment requires training from a member of the imaging facility staff, or be able to demonstrate previous microtome experience.
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Leica RM2155 Rotary Microtome
This fully automated rotary microtome has both a low profile disposable knife holder for paraffin sectioning, and a tungsten carbide knife for sectioning of resin embedded material. It is capable of routine sectioning between 3 - 50 microns. Use of this equipment requires training from a member of the imaging facility staff, or be able to demonstrate previous microtome experience. |
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Ventana 300 Rotary Microtome
This fully automated rotary microtome is equipped only with a low profile disposable knife holder for routine paraffin sectioning (3-50microns). Use of this equipment requires training from a member of the imaging facility staff, or be able to demonstrate previous microtome experience.
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Leica CM3050 Cryomicrotome
This fully automated cryostat has a fine orientation system to ensure high quality sectioning and consistant results. It can section fixed and unfixed tissues. Sample must be frozen and embedded in a freezing medium. The section thickness ranges between 0.5 - 300 microns on a specimen as large as 55 x 70 mm. Has programmable defrost cycles with a twin-compressor cooling system. Trained users are allowed to operate the cryostat.
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EM Preparation
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BAL-TEC HPM 010 High Pressure Freezing Machine
Performs cryofixation of suspensions, monolayer cell cultures and tissue. Fully automated with a one button operation. Freezes specimens up to 200 micron thickness and 2mm diameter without visible ice crystal damage. Does not require freeze protective additives. Real time temperature and pressure measurement digitally displayed. High freezing rate decreasing the resulting damaging crystal formation. |
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Leica EMTRIM Specimen Trimmer
Designed for rapid trimming of embedded samples and can be used for TEM, SEM, and Light Microscopy. Safer than trimming with a razor blade. Can trim samples as small as 0.2mm. Resin waste is removed by a vacuum cleaner. |
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Reichert UltracutE Ultramicrotome
Designed for the sectioning of ultra-thin EM samples. Can slice sections as thin as 5nm. Has programmable speed and feed combinations. Uses Diatome diamond knives for the smoothest possible cuts.
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Reichert EMM80 Slam Freezer
Used for plunge freezing, slam freezing, and spray freezing applications. Has a LN2 cooled gold-coated copper block. Ideal for cryofixation and immunolabeling without freeze damage |
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Leica EMKRM2 Glass Knife Breaker
Glass Knife Breaker produces 45 degree knives from 6.4, 8, and 10mm thick glass. |
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Leica EMAFS Freeze Substitution System
Designed for substitution and low temperature embedding of samples after cryofixation over a period of days. Fully programmable and customizable. Allows for UV polymerization of specimens in Lowicryl. |
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Denton Bench Top Turbo III Sputter Coater
Features Include rotary shadowing, carbon coating, heavy metal sputtering, glow discharging. This device is available to facility users for grid preparation. |
