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Imaging Core Facility
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Histological Preparation and Equipment - More Info
Procedure |
Cost |
| Tissue Processing |
1-30 Cassettes - $8.50/cassette
31+ cassettes - $7.00/cassette |
| Immunostaining |
$20.00/slide |
| Cryoembedding |
$10.00/block |
| Slides (H&E stained) - includes coverslipping |
$15.00/rack |
| Paraffin Microtome |
$17.50/hour |
| Cryomicrotome |
$20.00/hour |
| Giemsa staining |
$10.00/rack |
| Antigen Retrieval |
$10.00/run |
| PAS staining |
$6.00/slide |
- Tissue sections from paraffin microtome on appropriate slides. Each slide typically holds 3-6 sections.
- A rack carries up to 19 slides. H&E staining and coverslipping takes ~1.5 hours.
- Frozen tissue sections obtained from Leica CM3050 cryostat. One slide typically holds 3 sections.
EM Sample Preparation and Equipment - More Info
Procedure |
Cost |
| Plunge/Slam Freezing |
$40.00/hour |
| Spray Freezing |
$40.00/hour |
| High Pressure Freezing |
$130.00 setup + $40.00/hour |
| Freeze Substitution |
$12.00/vial |
| Standard Fixation/Embedding |
$32.00/vial |
| Ultramicrotome |
$24.00/hour |
| Cryoultramicrotome |
$45.00 setup + $40.00/hour |
| Heavy Metal Staining |
(up to 10 grids) $20.00/run |
| Immunogold Labeling |
$18.00/grid |
| Carbon Coating |
$21.00/run |
- Fixatives (aldehydes, OsO4), dehydrants and resins included in the cost. Processing time is 1-2 weeks.
- Diamond knife charges offset resharpening costs.
- Gold-labeled secondary antibodies supplied (see above) and heavy metal staining
Microscopy Equipment - More Info
Equipment |
Cost |
| Hitachi H7600 Transmission Electron Microscope |
$55.00/hour |
| Zeiss LSM-510 Laser Scanning Confocal Microscope |
$60.00/hour |
| Zeiss Axioplan 2i Upright Microscope |
$30.00/hour |
| Zeiss Axiovert 200m Inverted Microscope |
$30.00/hour |
| Olympus Dissection Microscope |
$15.00/hour |
| Zeiss Axiostar Brightfield Microscope |
$7.00/hour |
| TIRF Microscope |
$60/1st hour; $30/each additional hour |
| EM Negatives |
$25.00 setup + $1.00/negative |
Computers and Printers - More Info
Equipment |
Cost |
| 3d Imaging and Deconvolution Workstation |
$7.00/hour |
| Prints - Fujix Pictography photo printer |
$7.00 full/$3.00 half |
| Prints - Olympus Dye Sub (4" x 5") |
$1.00/print |
Imaging Staff Labor Charges
Labor Type |
Cost |
| Preparation |
$40/hour |
| Imaging |
$60/hour |
Imaging Facility Supplies
Item |
Retail Price |
| DataCDs/DVDs |
$4.00/$8.00 |
| Grid storage box |
$10.00 |
| Slide Box - cardboard (20 slides) |
$15.00 |
| Slide Box - plastic (25 slides) |
$8.50 |
| Slide Box - plastic (100 slides) |
$12.00 |
| Gelatin subbed slides |
$1.00/slide |
| Superfrost charges slides |
$30.00/box or $0.50/slide |
| DAPI |
$1.00/stock tube |
| For other items, please inquire in the Facility |
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Prices effective November 1, 2008, subject to change without notice
For academic charges click here.
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